RNA

Part:BBa_K2725016:Design

Designed by: Eva Steele   Group: iGEM18_Edinburgh_UG   (2018-10-11)


J23108 - supD - B1006


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 77
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 80


Design Notes

The Anderson promotor was positioned the same number of bases upstream of pLac promoter in BBa_K914000.


Source

The sequence of the supD element of this part came from BBa_K914000. The sequence for upstream J23108 Anderson promoter and downstream B1006 Terminator came from BBa_J23108 and BBa_B1006 respectively.

References